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Fig. 3. Detection of the 3xP3-white+ transgenesis marker. a) Schematic representation of the pBac-GbW-attB transgenesis vector. Indicated elements are not drawn to scale. pBac5′ and pBac3′, piggyBac 5′ and 3′ inverted terminal repeats. 3xP3: 3xP3-DmHsp70 artificial promotor. Gb-white CDS: G. rubens/ <t>G.</t> <t>bimaculatus</t> cDNA (see Fig. 1a). <t>MCS,</t> multiple cloning site. b) Images of control and transgenic nymphs with the indicated genotypes. Note the highly restricted Gb-w+ expression associated with the pBac-GbW-attB{2} insertion (arrow). Bars: 0.5 mm. c) Images of control and transgenic adult eyes of the same genotypes as above. Bar: 1 mm.
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Fig. 3. Detection of the 3xP3-white+ transgenesis marker. a) Schematic representation of the pBac-GbW-attB transgenesis vector. Indicated elements are not drawn to scale. pBac5′ and pBac3′, piggyBac 5′ and 3′ inverted terminal repeats. 3xP3: 3xP3-DmHsp70 artificial promotor. Gb-white CDS: G. rubens/ <t>G.</t> <t>bimaculatus</t> cDNA (see Fig. 1a). <t>MCS,</t> multiple cloning site. b) Images of control and transgenic nymphs with the indicated genotypes. Note the highly restricted Gb-w+ expression associated with the pBac-GbW-attB{2} insertion (arrow). Bars: 0.5 mm. c) Images of control and transgenic adult eyes of the same genotypes as above. Bar: 1 mm.
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Fig. 3. Detection of the 3xP3-white+ transgenesis marker. a) Schematic representation of the pBac-GbW-attB transgenesis vector. Indicated elements are not drawn to scale. pBac5′ and pBac3′, piggyBac 5′ and 3′ inverted terminal repeats. 3xP3: 3xP3-DmHsp70 artificial promotor. Gb-white CDS: G. rubens/ G. bimaculatus cDNA (see Fig. 1a). MCS, multiple cloning site. b) Images of control and transgenic nymphs with the indicated genotypes. Note the highly restricted Gb-w+ expression associated with the pBac-GbW-attB{2} insertion (arrow). Bars: 0.5 mm. c) Images of control and transgenic adult eyes of the same genotypes as above. Bar: 1 mm.

Journal: G3 (Bethesda, Md.)

Article Title: The white gene as a transgenesis marker for the cricket Gryllus bimaculatus.

doi: 10.1093/g3journal/jkae235

Figure Lengend Snippet: Fig. 3. Detection of the 3xP3-white+ transgenesis marker. a) Schematic representation of the pBac-GbW-attB transgenesis vector. Indicated elements are not drawn to scale. pBac5′ and pBac3′, piggyBac 5′ and 3′ inverted terminal repeats. 3xP3: 3xP3-DmHsp70 artificial promotor. Gb-white CDS: G. rubens/ G. bimaculatus cDNA (see Fig. 1a). MCS, multiple cloning site. b) Images of control and transgenic nymphs with the indicated genotypes. Note the highly restricted Gb-w+ expression associated with the pBac-GbW-attB{2} insertion (arrow). Bars: 0.5 mm. c) Images of control and transgenic adult eyes of the same genotypes as above. Bar: 1 mm.

Article Snippet: A 2,820 bp DNA fragment (in bold in Supplementary File S1) containing the 3xP3-DmHsp70 promoter, the G. rubens/G. bimaculatus Gb-w CDS, an attB site, and a multiple cloning site (MCS) was synthesized and cloned in the pXL-BACII-LoxP-3xP3-DsRed-LoxP plasmid (a gift from Christopher Potter; Addgene #26852) linearized with XhoI and EcoRI.

Techniques: Marker, Plasmid Preparation, Cloning, Control, Transgenic Assay, Expressing